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ASFV DNA current in non-biting flies from outbreak farms in Romania – Swine abstracts


African swine fever (ASF) is a extremely contagious and extreme haemorrhagic illness of Suidae, with mortalities that strategy 100%. A number of research recommended the potential implication of non-biting dipterans within the unfold of ASFV in pig farms as a result of identification of the ASFV DNA. Nonetheless, to our information, no research has evaluated the viral DNA load in non-biting dipterans collected in outbreak farms and no danger elements have been analysed. On this context, our research aimed to analyse the danger elements related to the presence of non-biting dipterans collected from ASF outbreaks in relation to the presence and cargo of viral DNA.


Strategies: Yard farms, sort A farms (medium-sized farms with some biosecurity procedures and able to delivering animals to business abattoirs), and business farms, had been focused for sampling in 2020. In 2021, no yard farms had been sampled. Every farm was sampled solely as soon as. The identification of the collected flies to household, genus, or species degree was carried out based mostly on morphological traits utilizing particular keys and descriptions. Swimming pools had been made previous to DNA extraction. All extracted DNA was examined for the presence of the ASFV utilizing a real-time PCR protocol. For this research, we thought-about each pattern with a CT worth of 40 as optimistic. The statistical evaluation was carried out utilizing Epi Information 7 software program (CDC, USA).


Outcomes: All collected non-biting flies belonged to 5 households: Calliphoridae, Sarcophagidae, Fanniidae, Drosophilidae, and Muscidae. Of the 361 swimming pools, 201 had been optimistic for the presence of ASFV DNA. The obtained CT values of the optimistic samples ranged from 21.54 to 39.63, with a median worth of 33.59 and a imply worth of 33.56. Considerably decrease CT values (equivalent to larger viral DNA load) had been obtained in Sarcophagidae, with a imply worth of 32.56; a considerably larger variety of optimistic swimming pools had been observed in August, imply worth = 33.12.


Conclusions: Our research brings compelling proof of the presence of the commonest synanthropic flies close to home pig farms carrying ASFV DNA, highlighting the significance of strengthening the biosecurity measures and protocols for prevention of the insect life cycle and distribution.

Balmoș OM, Ionică AM, Horvath C, et al. African swine fever virus DNA is current in non-biting flies collected from outbreak farms in Romania. Parasites Vectors. 2024; 17: 278. https://doi.org/10.1186/s13071-024-06346-x

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