Antimicrobial resistance is an growing problem in semen preservation of breeding animals, particularly within the porcine species. Micro organism are a pure part of semen, and their progress needs to be inhibited to guard sperm fertilizing capability and the feminine’s well being. In pig breeding, the place semen is routinely saved at 17°C within the liquid state, options to standard antibiotics are urgently wanted. Photodynamic inactivation (PDI) of micro organism is a well-established instrument in medication and the meals trade however this expertise has not been extensively adopted in semen preservation. The particular problem on this setting is to selectively inactivate micro organism whereas sustaining sperm integrity and performance.
Strategies: The goal of this examine was to check the precept of PDI in liquid saved boar semen utilizing the photosensitizer 5,10,15,20-tetrakis(N-methyl-4-pyridyl)-21H,23H-porphine (TMPyP) and a white mild LED-setup.
Outcomes: In step one, photophysical experiments comprising singlet oxygen phosphorescence kinetics of TMPyP and willpower of the photosensitizer triplet time revealed a sufficiently excessive manufacturing of reactive singlet oxygen within the semen extender, whereas seminal plasma acted as robust quencher. In vitro experiments with prolonged boar semen confirmed that the established PDI protocol preserves sperm motility, membrane integrity, DNA integrity, and mitochondrial exercise whereas effectively lowering the micro organism beneath the detection restrict. A proof-of-concept insemination examine confirmed the in vivo fertility of semen after photodynamic therapy.
Conclusion: Utilizing the PDI method, an modern instrument was established that effectively controls micro organism progress in prolonged boar and maintains sperm fertility. This might be a promising contribution to the One Well being idea with the potential to scale back antimicrobial resistance in animal husbandry.
Luther A-M, Varzandeh M, Beckermann C, Feyer L, Maaßen IK, Oldenhof H, Hackbarth S and Waberski D (2024) Fertility after photodynamic inactivation of micro organism in prolonged boar semen. Entrance. Microbiol. 15:1429749. https://doi.org/10.3389/fmicb.2024.1429749
