Investigating infectious brokers in porcine abortion materials and stillborn piglets poses challenges for practitioners and diagnostic laboratories.
Strategies: On this examine, pooled samples of particular person reference organs (thymus and coronary heart) from a complete of 1000 aborted fetuses and stillborn piglets have been investigated utilizing quantitative PCR protocols for porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) and porcine circovirus sort 2 (PCV2). Concurrently, a pluck-pool containing equal parts of fetal thymus, coronary heart, and lung tissue was collected, frozen at − 20 °C, and re-analyzed when a specific amount of both PRRSV-1 RNA or PCV2 DNA was detected in particular person reference organs.
Outcomes: 13 pluck-pools have been assessed for PRRSV-1, all being PCR-positive. For PCV2, 11 of 15 pluck-pools investigated have been PCR-positive. In all pluck-pools testing unfavorable, viral hundreds in particular person swimming pools have been low.
Conclusion: This examine signifies that pluck-pools will be precious diagnostic materials and the consolidation of a number of organs by way of a single RNA/DNA extraction optimizes the utilization of obtainable laboratory sources. Extra analysis is required to evaluate the feasibility of follow-up investigations and to precisely outline standards for interpretation of viral hundreds in a medical context.
Kreutzmann H, Unterweger C, Schwarz L, Dimmel Okay, Auer A, Rümenapf T, Ladinig A.
Pluck-pools as diagnostic samples for detecting porcine reproductive and respiratory syndrome virus and porcine circovirus sort 2 in porcine abortion materials and stillbirths.
The Veterinary Journal. 2024; 304: 106081.
https://doi.org/10.1016/j.tvjl.2024.106081.
